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One of the currently best described roles of Cl– in signaling is the modulation of the With-No-Lysine (K) (WNK) – STE20-Proline Alanine rich Kinase (SPAK)/Oxidative Stress Responsive Kinase 1 (OSR1) – Cation-Coupled Cl–Cotransporters (CCCs) cascade. The with no lysine [K] (WNK) pathway consists of the structurally unique WNK kinases, their downstream target kinases, oxidative stress responsive (OSR)1 and SPS/Ste20-related proline-alanine-rich kinase (SPAK), and a multitude of OSR1/SPAK substrates including cation chloride cotransporters. View Past. Nov 21, 2023. WNK signaling regulates several ion channels. Enter 5 Numbers (01 - 69) and 1 Powerball Number (01 - 26) Enter 5 Numbers (01 - 70) and 1 Mega Ball Number (01 - 25) Enter 5 Numbers (01 - 41) and 1 Mega Ball Number (01 - 06) Enter 5 Numbers (01 - 48) and 1 Lucky Ball Number (01 - 18) Search to see the winning Pick 3/Pick 4 numbers for the dates selected. WNK isoforms phosphorylate and activate the related SPAK (SPS1. 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